A method for identifying a subset of polynucleotides from a starting amount of polynucleotides corresponding to the human genome for in vitro determination of a severity of the host response of a patient.

摘要

The present invention relates to a method for identifying a subset of polynucleotides from a starting amount of polynucleotides corresponding to the human genome for in vitro determination of a severity of host response of a patient in an acute infectious and / or acute inflammatory state in one Wherein a subject is used which comprises a plurality of different gene probes representing the substantially total human genome, the subjects being classified into at least two of the clinically determined phenotype groups according to their status of infection and / or inflammation, the changes in gene expression signals Are statistically compared between the phenotype groups, and such gene probes are selected whose gene expression signals are statistically significantly changed between at least two phenotype groups. A master score is determined as a measure of the severity of the host response, and a number of polynucleotides, which are significantly reduced in comparison to the initial quantity, are identified by determining a score which does not exceed a predetermined deviation from the master score. This score can be used to diagnose, predict the development or monitoring of an acute infectious and / or inflammatory condition of a patient and / or the therapy course control and / or to focus control.