Method and device for tracking a movement of a particle, in particular a single molecule, in a sample

摘要

Method for following a movement of a particle (2) in a sample (3), wherein the particle (2) is caused to emit photons with light (4) and wherein photons emitted by the particle (2) are registered, characterized in that the light (4) is directed onto the sample (3) with an intensity distribution (18) having a spatially limited minimum (19) and that the minimum (18) is tracked in the particle (2) moving in the sample (3) in that the intensity distribution (18) is shifted relative to the sample (3) such that a rate of the photons emitted by the particle (2) remains minimal.