METHOD FOR IDENTIFYING MICROBE IN CLINICAL SAMPLING AND BANK OF MASS SPECTRA MADE BY MALDI-TOF MASS SPECTROMETRY

摘要

The present invention relates to a method for identifying a microbe in a clinical sample by comparing mass spectra obtained by MALDI-TOF mass spectrometry of a sample of a protein extract of said sample to be analysed containing one such microbe, by comparison with reference spectra, characterised in that it comprises the following steps: a) establishing a bank of reference spectra by MALDI-TOF mass spectrometry, performed on samples of protein extracts obtained according to the same protocol from clinical samples after microbial lysis, without isolating and/or without culturing said microbes; b) obtaining a spectrum of a sample to be analysed of protein extract obtained from a clinical sample of the same nature obtained according to the same protocol; and c) determining that said clinical sample from which a sample to be analysed has been obtained contains a given reference microbe selected among the various reference microbes of said reference bench if the spectrum of step b) to be analysed is found to contain all the spikes that characterise such a reference spectrum of a reference sample containing said given reference microorganism.