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PROTEIN PURIFICATION IN THE PRESENCE OF NONIONIC ORGANIC POLYMERS AT ELEVATED CONDUCTIVITY
PROTEIN PURIFICATION IN THE PRESENCE OF NONIONIC ORGANIC POLYMERS AT ELEVATED CONDUCTIVITY
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机译:电导率较高的非离子有机聚合物存在下的蛋白质纯化
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摘要
A method of purifying a desired protein from a preparation includes providing the preparation in a form having less than about 5% of the chromatin residing in the original production medium, contacting the preparation with a nonionic organic polymer in an amount sufficient to cause the desired protein to precipitate or adsorb on a nonionic hydrophilic surface, and adjusting a salt concentration before or during the contacting step, the adjusting step providing a sufficient salt concentration to produce a conductivity greater than physiological conductivity.
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