VARIANTS OF YEAST NDI1 GENE, AND USES THEREOF IN THE TREATMENT OF DISEASE ASSOCIATED WITH MITOCHONDRIAL DYSFUNCTION

摘要

A nucleic acid sequence encoding the yeast NDI1 protein of SEQ ID NO: 542 or a functional variant thereof having at least 90% sequence identity with SEQ ID NO: 2 is described. The nucleic acid sequence comprises at least 50 codons which are codon optimised compared with the sequence of yeast NDI1 gene of SEQ ID NO: 1. An immune optimised functional variant of the yeast NDI1 protein of SEQ ID NO: 542 having at least 90% sequence identity with SEQ ID NO: 542 is also described, and includes at least one amino acid change selected from the group consisting of L194F, K283E, K9R, S142N, L501M, L402I, A386S, S85K, F89H, L93M, K195E, L18M, K213E, K372E, L258F, K510E, L158M, R478Q, L482M.