ASSAY FOR DETECTING FRRE LIGHT CHAINS BY CAPILLARY ZONE ELECTROPHORESIS

摘要

The invention provides a method of detecting two different free light chains (FLCs) types comprising: (i) providing a sample from a subject; (ii) mixing the sample with two different anti-FLC antibodies, or fragments thereof, each antibody capable of specifically binding to a different FLC type to the other antibody and each antibody comprising a different fluorescent label, to form a mixture; (iii) passing the mixture through a capillary tube by capillary zone electrophoresis (CZE); and (iv) detecting the presence of the antibody or fragment thereof after passage through at least a portion of the capillary tube.