A short-wavelength laser-excitation chiral Raman Spectrometer (A short-wavelength laser-excitation Raman Optical Activity Spectrometer) comprises a laser-excitation light source between 325nm and 532nm, the linear polarized homogeneous system, circularly polarized light converter, sample cell holder, circularly polarized light splitter, double beam bifurcated optical fiber, Rayleigh line filter, imaging spectrometer, CCD sensitive to short-wavelength range and data processing system. The laser coming from laser-excitation light source goes through the linear polarized homogeneous system then exposures to the sample cell, or the laser coming from laser-excitation light source goes through the linear polarized homogeneous system and circularly polarized light converter then exposures to the sample cell. The left/right circularly polarized Raman signals coming from sample cell go through the Rayleigh line filter, then enter into the incidence end of Y-type double-beam optical fiber, divided into two beams of polarized light via circularly polarized light splitter, present as a linear array on the other end of the optical fiber, then enter into the incident slit of imaging spectrograph. The Raman signals are separated by the imaging spectrograph then enter the CCD on the exit of spectrograph which is sensitive in a wide range of short wavelength, and incident upon the top half and the bottom of it. CCD's acquisition system collects the light signals of the top half and the bottom half, that is, the right and the left ciculaly polarized Raman signals, converting them into electrical signals and transmitting the signals to the computer. After data processing, the sum of right and left circularly polarized Raman signals is counted as the total Raman signal, and the difference between right and left circularly polarized Raman signals as the circularly polarized Raman difference signal, that is, the Raman optical activity signal, and One acquisition when normalized difference of circular polarization intensity is computed as the circularly polarized Raman difference signal divided by the total Raman signal. The short-wavelength chiral Raman Spectrometer that can be applied to confirmation of chiral molecules' and biomolecules' absolute configurations, is a powerful tool for determination of absolute configuration and conformation of chiral molecules in wide region of chemistry, biology and medicine.
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