PHASE-CONTRAST MICROSCOPY METHOD, OPTICAL UNIT, AND PHASE-CONTRAST MICROSCOPE

摘要

A method for phase-contrast microscopy on a specimen, and the microscope and optical unit for carrying out the method, is provided. The specimen is arranged in a vessel having an object liquid, in which a component of an illumination beam, which serves as direct radiation and has not been diffracted by the sample, is applied to a phase-shifting element, is characterized in that the illumination beam is deflected before it enters the object liquid by way of an optical deflection element which is changeable in terms of position and/or shape so as to compensate for a change in the main radiation direction of the illumination beam that occurs due to optical refraction at the object liquid.