ISOLATION AND LONG-TERM CULTURING OF ESTROGEN RECEPTOR-POSITIVE HUMAN BREAST EPITHELIAL CELLS

摘要

The present invention describes methods for long-term culturing of ERpos cells, cell lines, and/or cell strains with exetended lifespan and/or cell strain as well as culture medium compositions. The invention further describes methods for isolating cells which may be used as starting point for long-term culturing of ERpos cells, cell lines, and/or cell strains with extended lifespan and/or cell strain. The invention further discloses various methods for generating ERpos tumorigenic cells, cell lines, and/or cell strains with extended lifespan and/or cell strain as well as various assays for their use.