LOOPED UVEX TYPE PROBES, PRIMERS AND A WAY OF TICK-TRANSMITTED PATHOGENS' DETECTION AND USE THEREOF IN OPTIMIZED PCR REACTION IN ORDER TO IDENTIFY AN AMPLIFIED GENETIC MATERIAL

摘要

The object of the invention relates to Looped UVEx type probe and its application, together with an appropriately designed pair of primers, in optimized PCR reaction (Polymerase Chain Reaction) with Hot Start Taq DNA Polymerase in order to: (i) enable quick detection of the PCR product by visual observation of the PCR mixture's color change under UV light, directly in the reaction tube; (ii) increase the specificity of standard PCR reaction; (iii) decrease the number of false positive results during identification of the amplified genetic material such as DNA of tick-transmitted pathogens.