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SIMULATED INTERNAL STANDARD METHOD, DEVICE AND APPLICATION FOR MASS SPECTROMETRY QUANTITATIVE ANALYSIS
SIMULATED INTERNAL STANDARD METHOD, DEVICE AND APPLICATION FOR MASS SPECTROMETRY QUANTITATIVE ANALYSIS
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机译:质谱定量分析的模拟内部标准方法,装置及应用
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摘要
Present invention discloses a pseudo internal standard method, device and application for mass spectrometry quantitative analysis. The method comprises steps: taking a standard, and formulating same into standard solutions with different concentrations by using a blank substrate of a sample to be tested, and then taking the same standard, and formulating same into internal standard solutions with one to several concentrations by using the blank substrate of the sample to be tested; processing the standard solutions and the internal standard solutions with different concentrations, through a sample processing steps, into standard samples and internal standard samples; successively injecting the standard sample and the internal standard sample into a chromatography-mass spectrometry system to be analyzed and measured; plotting a ratio of peak areas of the standard sample and the internal standard sample against the concentrations of the standard samples to establish a calibration curve of pseudo internal standard method quantification; and processing the unknown sample by the same sample processing steps then following the same steps as standard sample analysis to inject the sample to be detected and the internal standard into the chromatography-mass spectrometry system for analysis and measurement, the peak area ratio of the sample over the internal standard is then compared with the calibration curve to determine the concentration of the unknown sample.
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