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SUBTRACTIVE IMMUNOASSAY METHOD AND LATERAL FLOW IMMUNOCHROMATOGRAPHY ASSAY STRIP FOR PERFORMING THE METHOD
SUBTRACTIVE IMMUNOASSAY METHOD AND LATERAL FLOW IMMUNOCHROMATOGRAPHY ASSAY STRIP FOR PERFORMING THE METHOD
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机译:减法免疫分析方法和执行该方法的侧向流层析层析检测条
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摘要
A subtractive immunoassay for detecting the presence or absence of first and second analytes in a sample includes in the following order the steps of: a) conjugating a first detector antibody to the first analyte to tag the first analyte with a first detector tag; b) conjugating a first capture antibody to the first analyte to capture the first analyte to completely deplete the first analyte from the sample; c) conjugating a second detector antibody to the second analyte in the depleted sample to tag the second analyte with a second detector tag; d) conjugating a second capture antibody to the second analyte to capture the second analyte from the sample; e) detecting presence or absence of the first and second detector tags at the site of the first and second capture antibody, respectively, and thereby detecting the presence or absence of the first and second analytes in the sample.
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