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METHOD AND SCANNING FLUORESCENCE MICROSCOPE FOR MULTIDIMENSIONAL HIGH-RESOLUTION IMAGING OF A STRUCTURE OR A PATH OF A PARTICLE IN A SAMPLE
METHOD AND SCANNING FLUORESCENCE MICROSCOPE FOR MULTIDIMENSIONAL HIGH-RESOLUTION IMAGING OF A STRUCTURE OR A PATH OF A PARTICLE IN A SAMPLE
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机译:样本中结构或粒子路径的多维高分辨率成像的方法和扫描荧光显微镜
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摘要
For multi-dimensional high-resolution imaging a structure marked with fluorescence markers, fluorescence enabling light is focused to illuminate a measurement area in a sample. A partial area of the measurement area is subjected to fluorescence inhibiting light. The partial area omits a center of the measurement area in that an intensity distribution of the fluorescence inhibiting light comprises a line-shaped intensity minimum. A minimal extension of the intensity minimum in a direction through the center area is by a factor k≥2 smaller than a diameter of the measurement area in said direction. Without spatial resolution, fluorescence light emitted out of the measurement area is measured for a plurality of consecutive angle positions of the intensity minimum about the center, while the measurement area, for each angle position, is subjected to the fluorescence enabling light. A value of the measured fluorescence light is assigned to the position of the center.
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