Helicopter having an effect of reducing pain, active substance of Yamabushitake mycelium, method for preparing active substance, and pharmaceutical composition containing active substance

摘要

The present invention provides an active substance of Yamabushitake having an effect of reducing pain and a pharmaceutical composition containing the active substance. The active substance is prepared by the following steps. (A) inoculating Yamabushitake mycelium on an agar plate and culturing at a temperature of 15 to 32 ° C. for 8 to 16 days; and (b) the Yamabushitake mycelium cultured in step (a) in the medium in the flask. Inoculating and culturing at 20-30 ° C. and pH 4.5-6.5 for 3-5 days, and (c) inoculating the Yamabushitake mycelium cultured in step (b) into the medium in the fermentation tank And culturing at a temperature of 24 to 32 ° C. and a pH of 4.5 to 5.5 for 8 to 16 days to obtain a fermentation medium for the Yamabushitake mycelium, and (d) the Yamabushitake mycelium in step (c). Drying the body fermentation medium to obtain a powder of Yamabushitake mycelium. The powder of the Yamabushitake mycelium can be further extracted with an alcohol solvent to isolate and purify a new compound called heripene. [Selection] Figure 11