PROTEINS HAVING IMIDAZOLE DIPEPTIDE SYNTHESIS ACTIVITY AND IMIDAZOLE DIPEPTIDE PRODUCTION METHOD

摘要

PROBLEM TO BE SOLVED: To address the problem of simply providing a low cost imidazole dipeptide, to thereby provide proteins that produce imidazole dipeptides with high efficiency such as carnosine, anserine and balenine, and a production method using same.;SOLUTION: A modified protein comprises an L-amino acid α-ligase illustrated by sequence number 1 in which at least 1-3 amino acid residues of an amino acid sequence of YwfE are substituted wherein the asparagine (N) residue at position 108 from the N terminal is substituted for an alanine (A) residue, glutamic acid (E) residue or glutamine (Q) residue, or additionally, the isoleucine (I) residue at position 112 is substituted for a valine (V) residue, and/or the histidine (H) residue at position 378 is substituted for a lysine (K) residue or an arginine (R) residue.;SELECTED DRAWING: Figure 1;COPYRIGHT: (C)2018,JPO&INPIT