Staining quality assessment method and system for in situ hybridization and immunohistochemistry

摘要

Immunohistochemistry (IHC) and in situ hybridization (ISH) have the purpose of detecting, locating and quantifying certain analytes for various diagnostic purposes. The quality of staining analyzed can vary widely for a variety of reasons. Accordingly, the present invention provides a method and system for assessing staining quality and a method for establishing objective criteria for assessing staining quality and for applications in the field of in situ hybridization and immunohistochemistry. Provide a system. In one possible embodiment, the present invention separates multispectral image data of a tissue specimen to obtain specimen intensity images, each specimen intensity image comprising a signal from one staining, Calculating a metric based on the specimen intensity image, wherein the metric is uniformity, distribution, and / or dispersion of pixel intensity values in the specimen intensity image; and calculating the metric Evaluating the staining quality of a slide by comparing with a predetermined cut-off value for pixel intensity uniformity, distribution, and / or spread, wherein the calculated metric matches the predetermined cut-off value Or if it exceeds, the staining quality of the slide is evaluated as acceptable and the calculated metric is a predetermined cutoff If it does not match with the staining quality of the slide it is evaluated to be unacceptable, and a step. In order to establish objective criteria for assessing staining quality, in one possible embodiment, the method and system described above employs a metric calculated based on the specimen intensity image to determine a predetermined quantification of staining quality. Deriving cut-off values for pixel intensity uniformity, distribution, and / or scatteriness by combining with (pre-established) data. [Selection] Figure 14