Affinity chromatography purification method using low conductivity wash buffer

摘要

As used herein, a method for producing a bispecific antibody comprising a first antigen binding site that specifically binds to a first antigen and a second antigen binding site that specifically binds to a second antigen. (A) culturing a cell containing a nucleic acid encoding the bispecific antibody, (b) recovering the bispecific antibody from the cell or culture medium, (c) the bispecific Contacting the affinity antibody with the affinity chromatography material, (d) washing the affinity chromatography material with a low conductivity aqueous solution having a conductivity value of about 0.5 mS / cm or less, (e) from the affinity chromatography material. The method is reported to include recovering the bispecific antibody, (f) performing an additional chromatography step, thereby producing the bispecific antibody.