Multispectral laser imaging (MSLI) method and system for imaging and quantification of blood flow and perfusion

摘要

Some embodiments of the inventive concept provide a system that applies laser speckle imaging or laser Doppler imaging using two wavelengths that have different transmittances to the sample. The first of the two wavelengths is in the visible range with zero or very shallow penetration. This wavelength captures the tissue / organ surface anatomy and acts as a sample location marker, but does not capture subsurface blood flow and perfusion movements. The second wavelength is in the near infrared (NIR) range, which is much deeper penetration. This wavelength reveals the underlying blood flow physiology and is correlated with both sample movement and blood flow and perfusion movement. Thus, it is possible to extract the true blood flow and perfusion movements from the NIR imaging measurement results without being affected by the subject's movement artifacts.