Method of applying non-genetic material to implement site-specific modification of plant genome

摘要

The present invention discloses a method of applying non-genetic material to perform site-specific modification of plant genome. The method comprises: introducing a non-genetic material into the cell or tissue or plant part of the subject plant, wherein the non-genetic material is a nuclease specific for the targeting moiety or expresses the nuclease wherein the nuclease is a TALEN nuclease, a zinc finger nuclease (ZFN), a regularly spaced clustered clustered short palindromic repeats (CRISPR / Cas9) nuclease, or genomic editing It is any nuclease that can be realized. The nuclease serves to cleave the target moiety and then the site-specific modification of the target moiety is completed by DNA repair of the plant.