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Cell culture medium, culture method, and organoid
Cell culture medium, culture method, and organoid
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机译:细胞培养基,培养方法和类器官
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摘要
The present invention provides a cell culture medium with which serum-free long-term culture of an epithelial stem cell, an epithelial cancer cell, or a tissue containing at least one thereof can be achieved. The cell culture medium of the present invention includes: a Wnt agonist composed of a complex of Wnt protein and afamin, which is a substance capable of stabilizing the Wnt protein, and R-spondin; and at least one selected from the group consisting of a mitogenic growth factor, a bone morphogenetic protein (BMP) inhibitor, a transforming growth factor-² (TGF-²) inhibitor, and a p38 inhibitor.
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