Tissue sample fixation method using long immersion in aldehyde-based fixative solution

摘要

Including immersion in cold fixative solutions based on at least one aldehyde over a period longer than 2 days, followed by immersion in warm fixative solutions based on aldehydes. Improved histomorphology and IHC staining and improved preservation of post-translational modification signals such as biomarkers are achieved using the processes disclosed herein, as compared to standard room temperature fixation protocols. In addition, it is also possible to leave the tissue in a fixative solution for up to at least 14 days using these methods, which provides improved fixation compared to other protocols, Allows fixation to occur during transportation, transportation, and weekend or vacation while achieving results.