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Method for quantifying virus particles using capillary zone electrophoresis

机译:使用毛细管区带电泳定量病毒颗粒的方法

摘要

The present invention includes: a) introducing a biological sample containing virus particles into a capillary tube containing a buffer solution; and b) sufficient to allow separation of virus particles and other components in the sample. Applying an electric field of an appropriate voltage to the capillary tube to obtain an electrophoretic fraction; c) creating an electropherogram associated with the electrophoretic fraction; d) the electropherogram and a known concentration Determining the concentration of viral particles in the biological sample by comparing to an electropherogram made from a reference sample containing the viral particles, Provide a quantitative method.
机译:本发明包括:a)将含有病毒颗粒的生物样品引入含有缓冲液的毛细管中; b)足以分离样品中的病毒颗粒和其他成分。向毛细管施加适当电压的电场以获得电泳分数; c)创建与电泳分数相关的电泳图; d)电泳图和已知浓度通过与包含病毒颗粒的参考样品制成的电泳图进行比较,确定生物样品中病毒颗粒的浓度,提供一种定量方法。

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