Method using desmoglein I protein in soluble form for screening anti-aging activator

摘要

Use of at least one (non)complexed soluble peptide form, derived at least partly from a polypeptide of amino acid sequence encoded by a nucleic acid sequence of SEQ ID NO: 1 (comprising a fully defined 4512 base pairs as given in the specification) or its analog as a screening tool of active ingredient for preventing and/or treating skin aging, is claimed. Independent claims are included for: (1) process for screening of anti-aging active ingredients comprising: (a1) contacting at least one cell type capable of releasing at least one soluble peptide form with at least one anti-aging active ingredient to be tested, under conditions favorable for releasing soluble peptide form; (b1) determining a content of the soluble peptide form; and (c1) comparing the content determined in step (b1) at a content of the soluble form determined in the absence of chemical or biological compound to be tested; (2) cosmetic process to characterize the effectiveness of a cosmetic or therapeutic treatment to prevent and/or treat the signs of skin aging linked to chronological aging in an individual, comprising at least qualitative and quantitative characterization of the soluble form; and (3) non-therapeutic process for characterizing the effectiveness of a treatment capable of preventing and/or treating the signs of skin aging in an individual, comprising: (i) placing at least a first sample of skin surface representative of the person; (ii) quantifying the level of the sample particularly using enzyme-linked-immunosorbent assay technique, at least as complex form; (iii) repeating the steps (i) and (ii) on a second sample of skin surface representative of the individual; and (iv) comparing the results obtained from steps (ii) and (iii), preferably to derive information related to at least one effect of the treatment, where the first and second samples of skin surface correspond to different treatment steps. ACTIVITY : Dermatological. MECHANISM OF ACTION : None given.