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Rapid scanning of wide quadrupole RF windows while toggling fragmentation energy
Rapid scanning of wide quadrupole RF windows while toggling fragmentation energy
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机译:快速扫描宽四极杆RF窗口,同时切换碎片能量
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摘要
A sample is ionized using an ion source and the ion beam is received using a tandem mass spectrometer. An m/z range is divided into two or more precursor ion isolation windows. Two or more values for a fragmentation parameter are selected. A first value of the two or more values for the fragmentation parameter has a level that fragments a minimal amount of ions of the ion beam. The one or more additional values have increasingly aggressive levels that produce increasingly more fragmentation of the ions of the ion beam. For each precursor ion isolation window, the tandem mass spectrometer is instructed to perform a selection and fragmentation of the ion beam using the precursor ion isolation window and the first value and is instructed to perform one or more additional selections and fragmentations of the ion beam using the precursor ion isolation window and using the one or more additional values.
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机译:使用离子源将样品离子化,并使用串联质谱仪接收离子束。 m / z范围分为两个或多个前驱体离子隔离窗口。选择一个或多个碎片参数值。碎片参数的两个或更多个值中的第一值具有使离子束的最小量的离子碎片的水平。一个或多个附加值具有越来越高的侵略性水平,其产生越来越多的离子束离子碎片。对于每个前驱体离子隔离窗,指示串联质谱仪使用前驱体离子隔离窗和第一值对离子束进行选择和裂解,并指示对串联质谱仪使用前体离子隔离窗口,并使用一个或多个附加值。
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