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Compositions and cell-based methods for monitoring the activity of a Dengue Virus protease
Compositions and cell-based methods for monitoring the activity of a Dengue Virus protease
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机译:监测登革热病毒蛋白酶活性的组合物和基于细胞的方法
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摘要
The invention is directed to compositions, e.g., cell-based and multiplexed platforms, to screen for small molecule drugs that inhibit enzymes such as proteases, e.g., viral proteases, e.g., HIV proteases; and methods for making and using these compositions. The invention provides compositions and methods for identifying compositions, e.g., drug molecules, that can inhibit proteases, e.g., viral proteases such as HIV proteases. In alternative embodiments, the invention provides cell-based platforms or assays to screen for compositions, e.g., small molecules or drugs, that inhibit or modify the activity of enzymes such as calcium-dependent protein convertases involved in HIV envelope protein processing, including cleavage of the HIV gp160 envelope precursor, resulting in gp120 and gp41 envelope products. In one embodiment, the invention provides a cell-based or multiplexed platform for monitoring the activity of enzymes, e.g., proteases such as viral proteases.
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机译:本发明涉及组合物,例如基于细胞的平台和多重平台,以筛选抑制酶如蛋白酶如病毒蛋白酶如HIV蛋白酶的小分子药物。以及制备和使用这些组合物的方法。本发明提供了组合物和鉴定可以抑制蛋白酶如病毒蛋白酶如HIV蛋白酶的药物分子的组合物和方法。在替代实施方案中,本发明提供了基于细胞的平台或测定法,以筛选抑制或修饰酶(例如与HIV包膜蛋白加工有关的钙依赖性蛋白转化酶,包括裂解卵磷脂)的活性的组合物,例如小分子或药物。 HIV gp160包膜前体,产生gp120和gp41包膜产品。在一个实施方案中,本发明提供了一种基于细胞的或多重平台,用于监测酶例如蛋白酶如病毒蛋白酶的活性。
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