In the present invention, a method for the qualitative genetic characterization and/or gene expression characterization of predetermined cells in a fluid sample containing such cells is provided. The inventive method for the qualitative genetic and/or gene expression characterization of predetermined cells in a fluid sample containing such cells, comprises: a) selectively extracting at least a part of the predetermined cells from the sample forming a cell suspension cs0; and b) repeating the extraction step a) n times with the same sample of step a), with n≥1, forming at least one cell suspension csn; c) determining a gene expression profile gepn and/or a first copy number count cnc0 of at least one DNA and/or RNA with at least a part of the cell suspension cs0; d) determining at least one further gene expression profile gepn and/or a further copy number count cncn of at least one DNA and/or RNA with at least a part of at least one further cell suspension csn; e) calculating the predetermined cells' gene expression profile gep(P) of at least one predetermined DNA and/or RNA by subtracting gepn from gep, and/or the predetermined cells' copy number count cnc(P) of at least one predetermined DNA and/or RNA by subtracting cncn from cnc0; and f) evaluating the qualitative genetic and/or gene expression characteristics of the predetermined cells from gep(P) and/or cnc(P).
展开▼
机译:在本发明中,提供了一种用于对包含此类细胞的流体样品中的预定细胞进行定性遗传表征和/或基因表达表征的方法。本发明的用于定性分析含有这种细胞的流体样品中预定细胞的遗传和/或基因表达特征的方法,包括:a)从样品中选择性地提取至少一部分预定细胞,形成细胞悬液cs 0 Sub>; b)用步骤a)的相同样品重复提取步骤a)n次,n≥1,形成至少一个细胞悬液cs n Sub>; c)确定至少一个DNA和/或RNA与至少一部分细胞的基因表达谱gep n Sub>和/或第一拷贝数cnc 0 Sub>悬浮cs 0 Sub>; d)用至少一种其他细胞悬浮液csn的至少一部分确定至少一种DNA和/或RNA的至少一种其他基因表达谱gepn和/或另一种拷贝数计数cnc n Sub> ; e)通过从gep中减去gepn,和/或至少一种预定DNA的预定细胞的拷贝数cnc(P),计算至少一种预定DNA和/或RNA的预定细胞的基因表达谱gep(P)通过从cnc 0 Sub>中减去cncn来提取RNA和/或RNA; f)从gep(P)和/或cnc(P)评估预定细胞的定性遗传和/或基因表达特征。
展开▼