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Method of synthesis of gene library using codon randomization and mutagenesis
Method of synthesis of gene library using codon randomization and mutagenesis
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机译:利用密码子随机化和诱变合成基因文库的方法
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摘要
Proposed is a method of easily finding an error during analysis of various library sequences of nucleic acid base sequences after synthesizing a gene library using a combination of three nucleic acid base sequences (codon) translated into the same protein. This shows that it is possible to create a gene library having the same protein sequence but different nucleic acid base sequences. The present disclosure provides a novel experimental method capable of measuring a correlation between gene expression according to change in a codon by changing the usage of a codon optimized to express a specific gene in vivo.
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