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Device for simultaneous fluorescence contrasting effect in transmitted light and reflected light
Device for simultaneous fluorescence contrasting effect in transmitted light and reflected light
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机译:在透射光和反射光中同时产生荧光对比效果的装置
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摘要
The invention relates to a device for simultaneous fluorescence contrasting effect in transmitted light and reflected light, having a reflected light optical path for focusing of the excitation light via a lens onto a sample, having a fluorescence signal, which extends from the sample and is directed onto the same lens, having a dichroite, an emission filter, and a detection unit for the purpose of separating the excitation light from the fluorescence signal and for detection, having a luminescent layer behind the sample and a diaphragm for partial coverage of the excitation optical path between the sample and the luminescent layer, whereby a part of the excitation optical path, which impinges onto the luminescent layer, emits light, which irradiates the sample past the diaphragm by forming an oblique transmitted light illumination.
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