首页>
外国专利>
A Multi-Functional Peptide Benefiting Expression, Purification, Stabilization and Catalytic efficiency of Recombinant Proteins
A Multi-Functional Peptide Benefiting Expression, Purification, Stabilization and Catalytic efficiency of Recombinant Proteins
展开▼
机译:有益于重组蛋白表达,纯化,稳定和催化效率的多功能肽
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention relates to a multi-functional peptide benefiting expression, purification, stabilization and catalytic efficiency of recombinant proteins, which relates to the field of enzyme engineering and protein purification. The present invention provides a self-assembling amphipathic peptide, which fused with proteins including alkaline polygalacturonate lyase (PGL), lipoxygenase (LOX) and green fluorescent protein (GFP), which leads to successful purification by the nickel affinity chromatography with recovery rates were 47.01%, 39.01% and 56.1%, respectively. Furthermore, the expression quantity and thermostability of the three proteins were enhanced in different degree. Of which the half-life of the PGL-S1v1 and LOX-S1v1 were 2.3 and 3.8-fold as compared with the corresponding wild-type and the specific activity were 1.1 and 1.9-fold increase, respectively. The crude enzyme activity of PGL-S1v1 was 9-fold increase than the PGL.
展开▼