COMPOSITIONS AND METHODS FOR EFFICIENT GENE EDITING IN E. COLI USING GUIDE RNA/CAS ENDONUCLEASE SYSTEMS IN COMBINATION WITH CIRCULAR POLYNUCLEOTIDE MODIFICATION TEMPLATES

摘要

Compositions and methods are provided for genome modification of a target sequence in the genome of an Escherichia coli cell. The methods and compositions employ a guide RNA/Cas endonuclease system in combination with a circular polynucleotide modification template to provide an effective system for editing target sites within the genome of an Escherichia coli cell.