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Method to Detect/Identify Bacterial Species Using Flow Cytometry and Surface Enhanced Raman Scattering
Method to Detect/Identify Bacterial Species Using Flow Cytometry and Surface Enhanced Raman Scattering
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机译:流式细胞仪和表面增强拉曼散射检测/鉴定细菌种类的方法
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摘要
A method uses flow cytometry to prepare surface enhanced Raman scattering (SERS) substrates for obtaining SERS spectra of bacteria. The method involves using a flow cytometer to sort bacterial cells into populations of bacterial cells based upon their biophysical characteristics. The cells may then be washed with a borate buffer to remove any chemical species that degrade the SERS response. A colloid-coated bacteria suspension is then created by mixing one of the populations of bacterial cells with SERS-active colloidal particles. The colloid-coated bacteria suspension is incubated until the SERS-active colloidal particles partition through the capsule and bind to the cell wall for each bacterial cell in the colloid-coated bacteria suspension. The colloid-coated bacteria suspension is then disposed onto a filter and a SERS spectra of the colloid-coated bacteria suspension is obtained using a Raman spectrometer.
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