首页> 外国专利> GENETIC MARKER FOR DISCRIMINATING AND DETECTING CAUSATIVE BACTERIA OF FISH EDWARDSIELLOSIS AND STREPTOCOCCOSIS, AND METHOD FOR DISCRIMINATING AND DETECTING CAUSATIVE BACTERIA USING SAME

GENETIC MARKER FOR DISCRIMINATING AND DETECTING CAUSATIVE BACTERIA OF FISH EDWARDSIELLOSIS AND STREPTOCOCCOSIS, AND METHOD FOR DISCRIMINATING AND DETECTING CAUSATIVE BACTERIA USING SAME

机译:区分和检测鱼类爱德华氏菌和链球菌致病菌的遗传标记,以及使用相同方法鉴别和检测致病菌的方法

摘要

The present disclosure relates to genetic markers for discrimination and detection of bacteria causing Edwardsiellosis and Streptococcosis in fish, and a method for discriminating and detecting the bacteria using the same. A genetic marker for discrimination and/or detection of each of Edwardsiella tarda, Streptococcus iniae, Streptococcus parauberis and Lactococcus garvieae, which cause fish diseases, is selected and a peptide nucleic acid and a primer pair, which are specific for the genetic marker, are used to amplify and obtain melting curves having different fluorescences depending on bacterial species. Thus, bacteria that cause fish diseases can be discriminated and whether or not fish would be infected with the bacteria can be detected in a simple, rapid and accurate manner.
机译:本公开涉及用于区分和检测引起鱼类中爱德华氏菌和链球菌病的细菌的遗传标记,以及使用该遗传标记区分和检测细菌的方法。选择用于歧视和/或检测引起鱼类疾病的爱德华氏菌,猪链球菌,副链球菌加维乳杆菌的遗传标记,并选择一种肽核酸遗传标记特异的引物对和引物对用于扩增并获得取决于细菌种类具有不同荧光的解链曲线。因此,可以区分引起鱼类疾病的细菌,并且可以以简单,快速和准确的方式检测鱼类是否会被细菌感染。

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