METHOD OF DETECTING INVASIVE FUNGI ACCORDING TO MORPHOLOGY THEREOF BASED ON CONTRAST STAINING, AND KIT FOR SAME

摘要

A method of detecting invasive fungi according to morphology thereof based on contrast staining, including: sterilizing and storing the necessary equipment aseptically; drawing 1 ml of venous blood from a tested subject's elbow vein; dripping one drop of the venous blood, prior to coagulation, into an ampoule containing 0.8 ml of a detection reagent under an aseptic environment; gently shaking the ampoule until the drop of venous blood is evenly distributed; leaving the ampoule to stand for 20 minutes to form a stained solution; sterilizing or disinfecting a microscope slide and a cover slip; dripping one drop of the stained solution on the microscope slide prepared under aseptic condition; observing the sample sequentially with 4×, 10× and 40× objective lenses and a 100× oil-immersion lens; magnifying with a 5 million pixel eyepiece; displaying an image of the sample on computer screen using a high-resolution imaging software for observation and record.