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Host-associated DNA sequences, primers, and probes for PCR-based identification of dog fecal pollution sources
Host-associated DNA sequences, primers, and probes for PCR-based identification of dog fecal pollution sources
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机译:与宿主相关的DNA序列,引物和探针,用于基于PCR的狗粪污染源识别
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摘要
A method for detecting dog-fecal contamination in a sample, comprising assaying the sample using a nucleotide sequence based genetic assay which comprises contacting the sample with at least one nucleic acid molecule having the nucleic acid sequence shown in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11, the nucleic acid sequence being capable of binding to a nucleic acid sequence in the sample, and detecting binding of the nucleic acid molecule to the nucleic acid sequence in the sample, wherein a presence of binding is indicative of the presence of dog-fecal contamination in the sample; the nucleic acid molecules; and a kit comprising at least two of the above-described nucleic acid molecules.
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机译:一种用于检测样品中狗粪污染的方法,包括使用基于核苷酸序列的遗传测定法测定样品,该方法包括使样品与至少一个具有SEQ ID NO:1、2所示核酸序列的核酸分子接触。 3、4、5、6、7、8、9、10或11,该核酸序列能够与样品中的核酸序列结合,并检测该核酸分子与该核酸序列的结合。样品,其中结合的存在指示样品中狗粪污染的存在;核酸分子;试剂盒,其包含至少两个上述核酸分子。
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