METHOD FOR CONCENTRATING A CELL SUSPENSION COMPRISING A MUCILAGINOUS BIOMASS OF OLEAGINOUS YEASTS

摘要

A method for concentrating, in order to favour the subsequent extraction process of intracellular lipids, a cell suspension containing a biomass of oleaginous yeasts fermented in fermentation broth under conditions that allow the intracellular accumulation of lipids, where the biomass contains significant quantities of mucilaginous material. The method includes:;a) cultivating the oleaginous yeasts in a fermentation broth to obtain a cell suspension containing the mucilaginous biomass;;b) subjecting the cell suspension obtained from a) to heat treatment, at a temperature between 95° C. and 120° C. and to acid treatment, to obtain a treated cell suspension containing the mucilaginous biomass containing intact oleaginous yeast cells; and;c) concentrating the treated cell suspension obtained from b), by removing at least part of the fermentation broth to obtain a concentrated cell suspension.