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Assay for Trichomonas vaginalis by amplification and detection of Trichomonas vaginalis AP65-1 gene

机译：阴道毛滴虫 AP65-1基因的扩增和检测分析

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摘要

A region of the Trichomonas vaginalis AP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whether T. vaginalis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of T. vaginalis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA. 展开▼

机译：已确定阴道毛滴虫 AP65-1基因的一个区域，该区域可用于进行扩增测定以明确确定 T。阴道中存在。公开了可用于对该基因进行热链置换测定（tSDA）反应的寡核苷酸。所公开的寡核苷酸可以用于对多种I T菌株具有特异性的测定中。与其他微生物的基因组或人类DNA没有交叉反应。 展开▼

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公开/公告号US9988692B2

专利类型

公开/公告日2018-06-05

原文格式PDF

申请/专利权人 BECTON DICKINSON AND COMPANY;
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申请/专利号US201414576361

发明设计人 RAY A. MCMILLIAN;
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申请日2014-12-19

分类号C07H21/00;C07H21/04;C12Q1/68;C12P19/34;

国家 US

入库时间 2022-08-21 12:55:53

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