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Genetic loci associated with phenotypes of reproductive growth in soybean and Methods of use

机译:与大豆生殖生长表型有关的遗传基因座及其使用方法

摘要

Claim 1: a method for selecting a plant of soybeans or soybean Germplasm with one or more phenotypes of reproductive Growth; The Method comprises: (a) detecting LTo plant soybeans or soybean Germplasm from at least one allele of one or more markers loci within or linked to a quantitative trait locus (QTL) associated with the days until the iniCio of flowering, in which the first allele promotes Reproductive Growth phenotype of selected from the group consisting of Early Flowering and late Flowering.Where one or more loci and markers selected from the group consisting of: (1) a Marker locus located in chromosome flanked by an interval that includes the Marker locus and SCT and former _ 187Treme distal chromosome 18; (2) a Marker locus Genetic recombination is located at a distance of less than or equal to 5 cm of the F2 Marker Locus on chromosome 18; (3) located at a Marker LocusA recombinant Genetic distance less than or equal to 5 cm of the F5 locus marker on chromosome 18; (4) a Marker locus Genetic recombination is located at a distance of less than or equal to 5 cmThe F6 Marker Locus on chromosome 18; (5) a Marker locus Genetic recombination located at a distance of less than or equal to 5 cm F7 locus marker on chromosome 18; and (6) a Locus MarkOr Genetic recombination located at a distance of less than or equal to 5 cm Marker F8 Locus on chromosome 18; and (b) select the plant of soybeans or soybean Germplasm QEU comprises at least one allele of the stage (a), and thereforeSelect a plant of soybean Reproductive Growth phenotype with a First selected from the group consisting of Early Flowering and late Flowering.Claim 27: a plant or Germplasm of soybean Progeny produced by the method of claim 1, 9, 16, 25 and 26.Claim 34: a case for selecting at least one plant of soybeans through marker assisted selection of a QTL associated with days to FloweringComprising: (a) primers or probes for Detecting a polymorphism in the Genome of soybean, where the physical position of the polymorphism were selected from the group consisting 60.914.550 base Pairs (BP) on chromosome 18, 61.796.264 BP on chromosome 18, 60.840.873 BP on chromosome 18, 60.745.556 BP on chromosome 18, 61.963.221 BP on chromosome 18, 62.111.333 BP on chromosome 18, 61.948911 BP on chromosome 18, 61.948.986 BP on chromosome 18,And a combination of these; and (b) the instructions for using the primers or Probes to detect markers loci and loci markers correlate with the Expected Number of days from theEmbra until the beginning of Flowering.
机译:1。一种选择具有一种或多种生殖生长表型的大豆或大豆种质的植物的方法;所述方法包括:(a)从与直到开花开始的天数相关的定量性状基因座(QTL)内或与之相关的一个或多个标记基因座的至少一个等位基因中检测LTO植物大豆或大豆种质。等位基因促进选自早开花和晚开花的生殖生长表型。其中一个或多个基因座和标记选自:(1)位于染色体两侧的标记位点,其间隔包括标记位点和SCT和前_187Treme远端染色体18; (2)标记基因座遗传重组位于第18号染色体F2标记基因座的5 cm以内。 (3)位于标记基因座处的重组遗传距离小于或等于18号染色体上F5基因座标记的5厘米; (4)标记位点遗传重组位于小于或等于5cm的距离处18号染色体上的F6标记位点; (5)标记基因座遗传重组,位于18号染色体上小于或等于5cm F7基因座标记的位置。 (6)基因座标记或遗传重组位于18号染色体上小于或等于5cm标记F8基因座的距离; (b)选择大豆或大豆种质QEU包含至少一个(a)阶段的等位基因,因此选择大豆生殖生长表型的植物,其First选自早开花期和晚开花期。 27.一种通过权利要求1、9、16、25和26所述的方法产生的大豆后代的植物或种质。权利要求34:一种通过标记辅助选择与FloweringComprising的天数相关的QTL来选择至少一种大豆植物的情况。 :(a)用于检测大豆基因组中多态性的引物或探针,该多态性的物理位置选自第18号染色体上的60.914.550个碱基对(BP),第18号染色体上的61.796.264个BP, 18号染色体上的60.840.873 BP,18号染色体上的60.745.556 BP,18号染色体上的61.963.221 BP,18号染色体上的62.111.333 BP,18号染色体上的61.948911 BP,18号染色体上的61.948.986 BP,以及这些; (b)使用引物或探针来检测标记位点的说明和与从胚芽到开花开始的预期天数相关的基因座标记。

著录项

  • 公开/公告号AR107074A1

    专利类型

  • 公开/公告日2018-03-21

    原文格式PDF

  • 申请/专利权人 PIONEER HI-BRED INTERNATIONAL INC.;

    申请/专利号AR2016P103883

  • 发明设计人

    申请日2016-12-16

  • 分类号C12N15/29;A01H5;A01H5/10;C12N15/82;

  • 国家 AR

  • 入库时间 2022-08-21 12:54:16

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