method for in situ localized detection of rna, method for determining the presence and location of a genetic sequence, padlock probe set, use of probe set, kit and method for identifying a cell

摘要

patent summary: "method for in situ localized detection of mrna". The present invention relates to the detection of RNA in a cell sample. more particularly, the present invention relates to localized detection of RNA in situ. The method is based on converting rna to complementary dna prior to segmenting cdna with the probe (s) into padlock. padlock probe hybridization depends on the cdna nucleotide sequence that is derived from the corresponding nucleotide sequence of the target rna. rolling circle amplification of the subsequently circularized padlock probe produces a detectable rolling circle product. advantageously, this allows the RNA to be detected in situ.