FLAVIVIRUS DIAGNOSTIC SYSTEM DEVELOPMENT USING GOLD NANOBASTS

摘要

The model describes the development of functionalization methodologies of gold nanobasters (aunrs) with monoclonal antibodies specific for the viral structural protein “e” (envelope protein) and their use for direct detection of dengue virus (denv) virus and other flaviviruses. , such as zika virus and yellow fever virus, in solution. These nanoparticles can be adjusted for the detection of biomolecules such as proteins and antibodies bound to aunrs from reading their absorption spectrum. In this model, anti-denv or anti-flavivirus immunoglobulins are bound to nanobastods previously functionalized to polyethyleneimine and then incubated with virus-containing samples. Detection of viral particles in the solution is achieved by analyzing the solution's plasmon resonance profile change using an uv-vis scanning spectrometer. This diagnostic method has detection sensitivity and specificity, low production cost, does not require a qualified professional and can be faster, more accurate and more practical than existing dengue, zika and yellow fever direct diagnosis techniques.