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method for transforming a plant cell or plant tissue using agrobacterium, transgenic plant, transgenic cell or transgenic tissue, culture medium, and use of a method.
method for transforming a plant cell or plant tissue using agrobacterium, transgenic plant, transgenic cell or transgenic tissue, culture medium, and use of a method.
Compositions and methods for transforming plants, preferably monocotyledons, and even more preferably, sugarcane are provided. Transformation methods involve infection of plant tissue with agrobacterium, and co-cultivation using culture medium comprising high concentrations of gelling agent, with the result of inhibiting exacerbated bacterial growth and increasing transformation frequencies. The invention includes regenerating transformed plants, and transformed plants themselves.
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