Method for identification and relative quantification of nucleic acid sequence expression, splice variant, translocation, copy number, or methylation changes using nuclease, ligation, and polymerase reactions combined with carryover prevention

摘要

The present invention relates to methods and devices for identifying and quantifying, even in limited abundance, nucleotide base mutations, insertions, deletions, translocations, splice variants, myna variants, alternative transcripts, alternative start sites, alternative coding sequences , alternative non-coding sequences, alternative splices, exon insertions, exon deletions, intron insertions, or other genome-level rearrangement and / or methylated nucleotide bases.