anti-hcv antibody detection assays of an individual using ns3 capture peptides

摘要

The present invention provides methods, kits and compositions for detecting an individual's anti-hcv antibodies in a sample using ns3 capture peptides. In certain embodiments, at least two ns3 helicase capture peptides (ns3h) and at least two conjugated peptides (e.g., ns3h conjugated peptides) are used together, allowing a wide dynamic range of ns3 antibody detection. an individual in a one-step essay. in other embodiments, methods are provided for detecting ns3-specific antibodies of an individual without the use of a reducing agent. In some embodiments, an individual's ns3-specific antibodies are detected with a "double injection" of ns3 conjugated peptide (e.g., conjugated peptide added to a sample before and after washing).