Method for increasing the percentage of monomeric antibody Fab - dsFv species multimu00e9ricas

摘要

The present invention provides a method for increasing the percentage of monomer in a composition of molecules of antibody recombinantemente expressed characterised in that the molecule of antibody underWhere at least one FV with specificity for an antigen of interest comprising a VH and VL in the VH and VL said are connected directly or indirectly via one or more connectors and stabilizesBy a disulphide bond between them.The said method comprises: a) a thermal conversion step to maintain the composition comprising the antibody molecule at a temperature in the range of 30 to 60 u00b0 C for a period of at least 1 ho.RA, in which step (a) is carried out in the presence of a reducing agent or after treatment with a reducing agent.