Immunoglobulin heavy chain of recombinant antibodies, production method of conjugated and conjugated

摘要

The present invention relates to a recombinant heavy chain of immunoglobulin which comprises a domain VH, an area ch1.A region of articulation that goes to the cysteine more C terminal region of intermediate joint, a circuit of the combination of sortase, a domain is a field, CH2 and CH3.In the circuit of the combination of sortase () consists of at least 16 amino acids located between cysteine more C terminal region of articulation between and the sequence of consensus vfx1FPP (SEQ ID No. 2.In which X1 is l or R) in the terminal n of a CH2 domain of the heavy chain of immunoglobulin; (b) comprises a source of recognition of sortase consisting of the amino acid sequence (SEQ ID lpx1tx2(1) in which X1 can be any amino acid residue, and X2 is g or; (c) comprises the terminal n to the sequence of (b), at least two amino acids; (d) applies,C terminal to the sequence of (b).At least six amino acids; and (E) in which the terminals C and N of amino acids for the sequence of (b) are not cysteine and its use in combination to place on the basis of the enzyme sortase.