Cuba opaque to lyse, neutralize and run electrophoresis with agarose slides vertically, for the realization of the comet assay

摘要

Cuba opaque to use, neutralize and run electrophoresis with agarose slides vertically, for the realization of the comet assay.The patent of utility model belonging to the field of research or analysis of materials by the use of electrophoresis.Understood by the container forming substantially paralelepiledal (1) provided with closure cover (2) made preferably acrylic or similar black opaque material.In its internal longitudinal surfaces (3) are positioned vertical rails (4) that allow to put pieces of glass (L) in a vertical position.Properly apart so that there is no contact between them.In the prototype, built and tested, to Cuba (1) was capable of storing 30 slides (L) of glass of dimensions 76 x 26 mm size normally used for this kind of test.In the interior of the vessel (1), approximately 10 millimeters from the edge, is the surgical wire electrode (5) provided in the horizontal direction.It connects to the negative pole (6) located in the left side (7) of the piece, and even in the interior of the vessel (1), in its lower region.It is the electrode (8) that connects to the positive pole (9) is installed in the right side (10).In the lower portion of the left side (7) is located, the tap (11) for drainage of the solutions.