Construction of DNA encoding a fusion polypeptide fusion, fusion protein, transgenic organism, vector, process for producing a desired protein in a cell of the filamentous fungus.And method for cleaving a desired protein from a recombinant fusion polypeptide

摘要

The regions of Kex2 cleavage of recombinant fusion proteins.The present invention relates to a DNA construct comprising a Kex2 fusion region comprising a Kex2 site and a pre sequence of Kex2 site immediately 5 'to the website of Kex2,A fusion polypeptide, vectors and cells comprising the DNA construct fusion.Methods for producing desired proteins from cells of the filamentous fungus and methods to increase the secretion and / or cleavage of a target protein from a cell.