Isolated nucleic acid molecule that has a sequence of amino acid, polypeptide, a chimeric gene comprising a molecule of nucleic acid isolation, the host cell is transformed.Method for the production of 2 - butanol, method for production of isobutanol and method for the production of 1 - butanol

摘要

From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.