A method of colonizing a carrier comprising a layer of polyamide nanofibres by a population of bacteria of the Rhodococcus erythropolis strain

摘要

A method of colonizing a carrier comprising a layer of polyamide nanofibres by a population of bacteria of the Rhodococcus erythropolis CCM 2595ad strain in which the bacterial population is transferred to a sterile culture medium containing NaNO₃ at a concentration of 3 g/l, K₂HPO₄ at a concentration of 1 g/l, MgSO₄.7H₂O at a concentration of 0.5 g/l, KCl at a concentration of 0.5 g/l, FeSO₄.7H₂O at a concentration of 0.01 g/l, and phenol at a concentration of 0.3 g/l, with pH 7.0, and is cultured, wherein, upon reaching the exponential or stationary growth phase, this bacterial population is separated from the culture medium, washed with physiological saline and resuspended to a cell concentration corresponding to an optical density of 0.5. It is then separated from the physiological saline and inoculated into a fresh sterile culture medium of the same composition as in the first step, and, with this culture medium and carrier comprising a layer of polyamide nanofibres, it is placed in a reaction vessel of the biological reactor, where it is maintained in a movement relative to the carrier, wherein a natural bond between the cells of the bacterial or yeast population and the polyamide nanofibres of the carrier is formed. During this procedure, the layer of polyamide nanofibres of the carrier is colonized in 48 hours by a bacterial population capable of further reproduction in an amount of at least 0.05 mg/cm² of the carrier.