A NEW HIRUSTELLA THOMPSONII BASED BIO-PESTICIDE AND A METHOD FOR DEVELOPMENT THEREOF

摘要

A method of manufacturing Hirsutella thompsonii using submerged fermentation is described here. The present invention relates to growing Hirsutella thompsonii in liquid formulation and its methods of scale up and mass multiplication. In present invention the fungal strain was also studied for the impact of different factors responsible for its optimum growth viz. carbon, nitrogen contents, temperature, pH, agitation and aeration etc. The study also confirms the uniformity in scale up and production process with maximum yield of 5.0—109 CFU/ml of fermented broth. The media for growth was optimized on the basis of addition and substitution of different organic and inorganic components. The optimum growth was obtained at temperature range 28 to 30° C and a pH from about 4.5 to 6.5. The invention also summarizes the most effective application methodology for effectively control the pest particularly mites population under field conditions.