PHARMACEUTICAL FORMULATION OF ANEMONE OBTUSILOBA D. DON. FOR HEPATOPROTECTIVE ACTIVITY AND METHODS THEREOF

摘要

This invention aimed to assess the hepatoprotective activity of ethanol (90%) extract Anemone obtusiloba D Don. stems on carbon tetrachloride (CCl4) induced hepatotoxicity in rats and to ascertain the claim of its use in liver disorders. Pre-treatment of rats with doses of 200 and 400 mg/kg, b.wt; p.o. of the ethanol extract significantly (P 0.05) lowered serum transaminases (AST and ALT), alkaline phosphatase (ALP), total bilirubin (TB) and increased the levels of the total proteins (TP) and albumin (ALB) respectively, in a dose dependent manner against the significant (P 0.01) alteration of these damaged marker enzymes when challenged with CCl4 (0.5 ml/kg, i.p.). Parallel to these changes, the stems extract prevented CCl4 induced oxidative stress by significantly restoring the levels of reduced glutathione and lowering the levels of hepatic malondialdehyde by inhibiting the production of free radicals. These biochemical parameters were supplemented by histopathological examination of liver sections. Taken collectively, these findings suggest that ethanol (90%) extract revel more significant (P 0.05) hepatoprotective potential against CCl4 induced hepatotoxicity in rats and confirms the folklore use of this plant